TB-500 (5mg)

Shop high-purity TB-500 at Eternal Peptides, the trusted source for verified research compounds in the USA. This synthetic Thymosin Beta-4 analogue is synthesized to a standard of ≥99% purity, with every batch rigorously tested and certified by Janoshik Analytical. TB-500 is a primary reagent for researchers investigating actin sequestration, cellular migration, and angiogenesis signaling. Order today for fast, secure US shipping and free Priority delivery on all orders over $200.

What is TB-500?

TB-500 is a synthetic research peptide modeled after thymosin beta-4 (Tβ4), a naturally occurring 43–amino acid peptide widely found in mammalian tissues. 

Structurally, TB-500 is considered a synthetic analogue or fragment-based derivative of thymosin beta-4 and is designed to replicate key biological activities observed in the endogenous peptide. Thymosin beta-4 was originally identified as an intracellular actin-binding protein involved in cytoskeletal organization and cellular migration.

In scientific literature, TB-500 and thymosin beta-4 are primarily investigated for their roles in tissue repair, wound healing, angiogenesis, and modulation of inflammatory responses. Existing research derived mainly from in vitro experiments and animal models suggests that TB-500 has potentially beneficial applications in musculoskeletal, cardiovascular, and epithelial tissue research

Mechanistically, research suggests that TB-500–related peptides influence actin dynamics, support cell migration, and interact with pathways involved in angiogenesis and inflammatory regulation.

Please note that these findings should be interpreted strictly as preclinical research, and TB-500 has not been approved for any human or veterinary clinical or therapeutic uses.

How TB-500 Works (Mechanism of Action)

TB-500 is a pleiotropic research peptide, meaning it appears to influence multiple biological pathways simultaneously.

Current mechanistic insights are derived primarily from in vitro experiments and animal models, where TB-500–related activity is studied in the context of cellular repair, inflammation, and tissue remodeling.

As we get into the mechanisms, it’s important to note that the synthetic TB-500 is thought to mimic the naturally found thymosin beta-4 very closely, so study results of the natural peptide may be taken to apply to the synthetic TB-500 within the scope of studies referenced here.

Actin Binding and Cell Migration

One of the most widely studied mechanisms associated with TB-500 is its relationship to actin regulation. As a thymosin beta-4–derived analogue, TB-500 is linked to actin sequestration, a process that helps regulate cytoskeletal dynamics inside the cell[1]. Actin remodeling is essential for cell shape changes, migration, and division.

In preclinical models, this actin-related activity is associated with enhanced cellular mobility, particularly in fibroblasts, endothelial cells, and progenitor cells. Cell migration is a foundational step in tissue repair because it enables cells to move toward sites of injury or structural disruption, supporting coordinated regeneration processes.

Angiogenesis and Vascular Support

Research also suggests that TB-500–related peptides influence angiogenic signaling, the process by which new blood vessels form from existing vasculature[2]. Angiogenesis is critical in tissue repair models because newly formed vessels improve oxygen and nutrient delivery to regenerating tissue.

This pathway is frequently studied in conjunction with BPC 157 to investigate synergistic effects on vascular growth and soft tissue recovery. Animal and cell-based studies indicate that thymosin beta-4 activity may upregulate pro-angiogenic factors while supporting endothelial cell survival.

Inflammatory Modulation

TB-500 has been examined for its potential role in modulating inflammatory signaling in preclinical research settings[2]. Inflammation is a necessary component of tissue response, but excessive or prolonged inflammatory activity can impair structural recovery.

Experimental findings suggest that TB-500–associated pathways may influence cytokine signaling and immune cell behavior, contributing to a more regulated inflammatory profile in damaged tissues[2]. By supporting balance rather than suppression, this mechanism is thought to help maintain conditions favorable for repair without disrupting normal immune surveillance in research models.

Cytoprotection and Tissue Remodeling

Another key area of investigation involves cytoprotective and remodeling-related effects. TB-500–linked activity has been associated with reduced apoptosis in stressed cells and improved survival signaling in environments exposed to mechanical or oxidative stress.

Additionally, studies suggest involvement in extracellular matrix organization and collagen alignment, both of which are essential for restoring tissue integrity after injury. Proper remodeling ensures that repaired tissue regains structural coherence rather than forming disorganized or fibrotic patterns, an outcome that is closely studied in musculoskeletal and connective tissue research models.

Note: All described mechanisms reflect preclinical research observations and should be interpreted strictly within experimental and laboratory contexts.

TB-500 Research Value

TB-500 is studied in research to explore how cells migrate, repair structural damage, and respond to physical stress at the tissue level.

Key areas of research include:

• Studying tissue repair signaling, often described in lay terms as how cells move to damaged areas and support recovery processes
• Exploring muscle and connective tissue models, relevant to research on flexibility, strain response, and structural integrity
• Investigating cell migration and actin regulation, which helps explain how cells reorganize during injury or stress
• Examining angiogenesis-related pathways, commonly framed as how new blood vessels may form in response to tissue demand
• Researching inflammatory modulation, focusing on how cellular signaling changes during tissue stress or damage
• Modeling systemic healing responses, where effects are observed beyond the immediate site of experimental injury

TB-500 Peptide Characteristics

Batch: EP-250421-TB05

Handling & Storage Guidelines

Store TB-500 vials (lyophilized form) at frozen temperatures, ideally at –4°F to –112°F (–20°C to –80°C), and protected from light and moisture to preserve stability.

When reconstitution is required, use bacteriostatic water and introduce the solvent gently to avoid foaming or agitation. Ideally, you should aliquot reconstituted solutions into single-use volumes to minimize repeated freeze–thaw cycles, which may affect peptide integrity. 

Short-term working solutions may be stored refrigerated at 36°F to 46°F (2°C to 8°C) for limited durations, while long-term storage should remain frozen. All handling should follow standard laboratory safety practices and institutional protocols.

COA / Quality Assurance

Eternal Peptides provides a Certificate of Analysis (COA) for every TB-500 production lot to support transparency and research reproducibility. COAs typically document peptide identity confirmed by analytical methods such as HPLC and/or mass spectrometry, verified purity levels, and testing for sterility and endotoxin content where applicable.

Additional data may include heavy metal screening and recommended storage conditions. All analytical testing is performed by independent third-party laboratories, usually Janoshik, to ensure reliable and unbiased verification. 

Check out the COAs on our Lab Tests page or request additional safety and compliance information from our support team.

Legal Disclaimer

Eternal Peptides sells TB-500 strictly for laboratory research use only. It is not approved for human or veterinary use and is not intended for clinical administration, therapeutic intervention, or diagnostic procedures of any kind.

The safety and efficacy of TB-500 in humans have not been established. As the purchaser, you  are solely responsible for ensuring compliance with all applicable local laws, regulations, and institutional biosafety or research-use policies.

Any misrepresentation of intended use or diversion from legitimate research applications may result in regulatory action or legal consequences.

Scientific References

1. Xiong Y, Mahmood A, Meng Y, Zhang Y, Zhang ZG, Morris DC, Chopp M. Neuroprotective and neurorestorative effects of thymosin β4 treatment following experimental traumatic brain injury. Ann N Y Acad Sci. 2012 Oct;1270:51-8.

https://pmc.ncbi.nlm.nih.gov/articles/PMC3547647/ 

2. Maar K, Hetenyi R, Maar S, Faskerti G, Hanna D, Lippai B, Takatsy A, Bock-Marquette I. Utilizing Developmentally Essential Secreted Peptides Such as Thymosin Beta-4 to Remind the Adult Organs of Their Embryonic State-New Directions in Anti-Aging Regenerative Therapies. Cells. 2021 May 28;10(6):1343.

https://pmc.ncbi.nlm.nih.gov/articles/PMC8228050/ 

3. Wang YY, Zhu QS, Wang YW, Yin RF. Thymosin Beta-4 Recombinant Adeno-associated Virus Enhances Human Nucleus Pulposus Cell Proliferation and Reduces Cell Apoptosis and Senescence. Chin Med J (Engl). 2015 Jun 5;128(11):1529-35.

https://pmc.ncbi.nlm.nih.gov/articles/PMC4733779/